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KMID : 0545120090190040351
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 4 p.351 ~ p.357
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Comparative Proteome Analysis of Two Antagonist Bacillus subtilis Strains
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Zhang C.X.
Zhao X.
Han F.
Yang M.F.
Chen H.
Chida T.
Shen S.H.
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Abstract
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Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB- 1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-1, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic diferrences between the antagonism of KB-1111 and KB-1122.
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KEYWORD
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Bacillus subtilis, Magnaporthe grisea P131, in vitro antifungal activity, proteomics, 2DE-PAGE
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